Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Niemann Pick C2 antibody (ab92736)

Key features and details

  • Rabbit polyclonal to Niemann Pick C2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Niemann Pick C2 antibody
    See all Niemann Pick C2 primary antibodies

  • Description

    Rabbit polyclonal to Niemann Pick C2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Niemann Pick C2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93309)

  • Positive control

    • This antibody gave a positive signal in human testis tissue lysate.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Chimpanzee
Monkey
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis.

  • Tissue specificity

    Epididymis.

  • Involvement in disease

    Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood.

  • Sequence similarities

    Belongs to the NPC2 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • EDDM1 antibody
      • Epididymal protein 1 antibody
      • Epididymal secretory protein antibody
      • Epididymal secretory protein E1 antibody
      • hE1 antibody
      • Human epididymis-specific protein 1 antibody
      • Niemann-Pick disease type C2 antibody
      • Niemann-Pick disease type C2 protein antibody
      • NPC intracellular cholesterol transporter 2 antibody
      • NPC2 antibody
      • NPC2_HUMAN antibody
      • Tissue specific secretory protein antibody
      see all

    Images

    • Western blot - Anti-Niemann Pick C2 antibody (ab92736)
      Western blot - Anti-Niemann Pick C2 antibody (ab92736)
      Anti-Niemann Pick C2 antibody (ab92736) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 minutes


      Human Epididymal secretory protein E1 precursor (Niemann pick C2)contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab92736)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab92736)
      IHC image of Niemann Pick C2 staining in human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 92736, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    References (0)

    Publishing research using ab92736? Please let us know so that we can cite the reference in this datasheet.

    ab92736 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-Niemann Pick C2 antibody (ab92736)
      Western blot - Anti-Niemann Pick C2 antibody (ab92736)
      Anti-Niemann Pick C2 antibody (ab92736) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 minutes


      Human Epididymal secretory protein E1 precursor (Niemann pick C2)contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab92736)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab92736)
      IHC image of Niemann Pick C2 staining in human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 92736, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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