Anti-BrdU antibody - Proliferation Marker (ab1893)
Key features and details
- Sheep polyclonal to BrdU - Proliferation Marker
- Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-BrdU antibody - Proliferation Marker
See all BrdU primary antibodies -
Description
Sheep polyclonal to BrdU - Proliferation Marker -
Host species
Sheep -
Tested applications
Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
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General notes
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.60
Constituent: 0.4% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893) Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893) This image is courtesy of an anonymous Abreviewab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
