HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
![HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)](https://www.abcam.com/ps/products/220/ab220507/Images/ab220507-281821-anti-brdu-antibody-bu1-75-icr1-hrp-immunohistochemistry.jpg "HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)")
Key features and details
- HRP Rat monoclonal [BU1/75 (ICR1)] to BrdU
- Suitable for: IHC-P
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Overview
-
Product name
HRP Anti-BrdU antibody [BU1/75 (ICR1)]
See all BrdU primary antibodies -
Description
HRP Rat monoclonal [BU1/75 (ICR1)] to BrdU -
Host species
Rat -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- IHC-P: BrdU treated rat skin tissue sections
-
General notes
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available in "Neuroscience protocols" on our "Protocol and troubleshooting tips" webpage.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
BU1/75 (ICR1) -
Isotype
IgG2a -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded BrdU treated rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)](https://www.abcam.com/ps/products/220/ab220507/Images/ab220507-281820-anti-brdu-antibody-bu1-75-icr1-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)Negative IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded normal rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
