Anti-BrdU antibody (ab152095)
Key features and details
- Rabbit polyclonal to BrdU
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-BrdU antibody
See all BrdU primary antibodies -
Description
Rabbit polyclonal to BrdU -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU.
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Positive control
- ICC/IF: HeLa cells treated with 100M BrdU for 4hrs. IHC-P: mouse BrdU treated intestine Flow Cyt: HeLa treated with 10 M BrdU for 30 minutes
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.
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ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.