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Anti-BrdU antibody (ab152095)

Price and availability

324 988 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-BrdU antibody (ab152095)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BrdU
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-BrdU antibody
    See all BrdU primary antibodies
  • Description

    Rabbit polyclonal to BrdU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to BrdU.

  • Positive control

    • ICC/IF: HeLa cells treated with 100M BrdU for 4hrs. IHC-P: mouse BrdU treated intestine Flow Cyt: HeLa treated with 10 M BrdU for 30 minutes
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)

    HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.

  • Flow Cytometry - Anti-BrdU antibody (ab152095)
    Flow Cytometry - Anti-BrdU antibody (ab152095)

    ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4%  paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
    Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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