Anti-CD44v6 antibody [VFF-7] (ab30436)
![Anti-CD44v6 antibody [VFF-7] (ab30436)](https://www.abcam.com/ps/products/30/ab30436/Images/ab30436-269699-anti-cd44v6-antibody-vff-7-immunohistochemistry.jpg "Anti-CD44v6 antibody [VFF-7] (ab30436)")
Key features and details
- Mouse monoclonal [VFF-7] to CD44v6
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD44v6 antibody [VFF-7]
See all CD44v6 primary antibodies -
Description
Mouse monoclonal [VFF-7] to CD44v6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Fusion protein corresponding to Human CD44v6. Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Database link: P16070 -
Epitope
This antibody specifically recognises an epitope encoded by exon v6 on the variant portion of human CD44. -
Positive control
- WB: HeLa and A549 cell lysates. IHC-P: Human breast cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
VFF-7 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [VFF-7] (ab30436)Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with ab30436 at 1/200 dilution.
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![Western blot - Anti-CD44v6 antibody [VFF-7] (ab30436)](https://www.abcam.com/ps/products/30/ab30436/Images/ab30436-400167-anti-cd44v6-antibody-vff7-western-blot-hela-wt-hela-cd44-knockout-a549-lncap.jpg)
Western blot - Anti-CD44v6 antibody [VFF-7] (ab30436)All lanes : Anti-CD44v6 antibody [VFF-7] (ab30436) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CD44 knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : LnCAP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab30436 observed at 80 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab30436 was shown to react with CD44v6 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab30436 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
