Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 110 563 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [2F10] to CD44v6
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-CD44v6 antibody [2F10]
See all CD44v6 primary antibodies
Description

Mouse monoclonal [2F10] to CD44v6
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human CD44v6.
Database link: P16070

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human breast carcinoma, skin and breast tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

2F10
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] (ab254174)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling CD44v6 with ab254174 at 3.73µg/ml, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] (ab254174)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD44v6 with ab254174 at 3.73µg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] (ab254174)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44v6 with ab254174 at 3.73µg/ml, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human skin tissue is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD44v6 antibody [2F10] (ab254174)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com