Anti-CD44v6 antibody [2F10] (ab254174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2F10] to CD44v6
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD44v6 antibody [2F10]
See all CD44v6 primary antibodies -
Description
Mouse monoclonal [2F10] to CD44v6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Recombinant fragment corresponding to Human CD44v6.
Database link: P16070 -
Positive control
- IHC-P: Human breast carcinoma, skin and breast tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F10 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling CD44v6 with ab254174 at 3.73µg/ml, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD44v6 with ab254174 at 3.73µg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44v6 with ab254174 at 3.73µg/ml, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human skin tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -