Arginine Assay Kit (Fluorometric) (ab252892)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Other biological fluids, Serum, Tissue
Overview
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Product name
Arginine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Other biological fluids, Tissue -
Assay duration
Multiple steps standard assay -
Product overview
Arginine Assay Kit (Fluorometric) ab252892 provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In our enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable fluorescence signal (Ex/Em = 535/587 nm). The kit is simple to perform, specific, high-throughput adaptable and sensitive– it can detect as low as 100 pmol/well of L-arginine in human biofluids and other biological samples.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Arginine Assay Buffer 1 x 25ml Arginine Converter Mix 1 vial Arginine Developer Mix 1 vial Arginine Enzyme Mix 1 vial Arginine Probe (in DMSO) 1 x 220µl Arginine Signal Mix 1 vial Arginine Standard 1 vial -
Research areas
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Relevance
Arginine is an alpha amino acid. The L form is one of the 20 most common natural amino acids. In mammals, arginine is classified as a semi essential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. -
Alternative names
- Arg
- L arginine
Images
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Example Data
Arginine standard curve.
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Example DataSpecificity of the detection of arginine over other amino acids: Other amino acids were tested at a 10-fold molar excess (each AA: 10 nmol) vs Arginine (1 nmol).
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Example DataEstimations of Arginine in human serum and plasma sample (4 µL each well) and rat brain (5.5 µg protein). L-arginine concentrations were 245 and 66.1 µM in human serum samples, 85.3 µM in human plasma, and 95.8 pmol/µg-protein in rat brain.