Anti-CYLD antibody (ab137524)
Key features and details
- Rabbit polyclonal to CYLD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CYLD antibody
See all CYLD primary antibodies -
Description
Rabbit polyclonal to CYLD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human CYLD aa 896-906.
Sequence:GGQNGFNIPQV
Database link: Q9NQC7 -
Positive control
- ICC/IF: A431 cells. IHC-P: Human gastric carcinoma tissue. WB: HepG2 whole cell lysate. Rat brain lysate. C8D30, NIH-3T3 , BCL-1 , Raw264.7 , C2C12 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYLD antibody (ab137524)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labelling CYLD with ab137524 at 1/100 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling CYLD with ab137524 at 1/200 dilution. The lower image is merged with DNA probe.
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Anti-CYLD antibody (ab137524) at 1/500 dilution + Rat brain lysate at 50 µg
Predicted band size: 107 kDa7.5% SDS-PAGE
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Anti-CYLD antibody (ab137524) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE -
All lanes : Anti-CYLD antibody (ab137524) at 1/1000 dilution
Lane 1 : C8D30 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : Raw264.7 whole cell lysate
Lane 5 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa7.5% SDS-PAGE