Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)

Price and availability

298 185 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP42Y] to p53 (phospho S46)
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

You may also be interested in

Product image
Anti-FAF1 antibody [EPR14754] (ab183045)
Product image
Anti-SPG48 antibody (ab224153)
Product image
Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)
Sodium nitroprusside, Vasodilator (ab145732)

Overview

  • Product name

    Anti-p53 (phospho S46) antibody [EP42Y]
    See all p53 primary antibodies
  • Description

    Rabbit monoclonal [EP42Y] to p53 (phospho S46)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human ovarian adenocarcinoma. WB: HepG2 cell lysate treated with etoposide.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP42Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Images

  • Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    All lanes : Anti-p53 (phospho S46) antibody [EP42Y] (ab76242) at 1/5000 dilution

    Lane 1 : Untreated HepG2 whole cell lysate
    Lane 2 : HepG2 whole cell lysate, treated with etoposide
    Lane 3 : HepG2 whole cell lysate, treated with etoposide, then the membrane was treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 1 minute


    Blocking buffer: 2% BSA/TBST
    Dilution buffer: 2% BSA/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)

    Immunohistochemical analysis of human ovarian adenocarcinoma using ab76242 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)

    Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab76242 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000).

    Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP.

    ab179477 (1/500) was used as a Pan control for ab76242. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells.

  • Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    All lanes : Anti-p53 (phospho S46) antibody [EP42Y] (ab76242) at 1/5000 dilution

    Lane 1 : HEK293 cells were treated with Etoposide whole cell lysates with p53 phospho S46 peptide
    Lane 2 : HEK293 cells were treated with Etoposide whole cell lysates with p53 non-phospho peptide
    Lane 3 : HEK293 cells were treated with Etoposide whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 8 seconds


    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    All lanes : Anti-p53 (phospho S46) antibody [EP42Y] (ab76242) at 1/5000 dilution

    Lane 1 : HepG2 whole cell lysate - treated with etopside
    Lane 2 : HepG2 whole cell lysate - untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)
    Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-p53 (phospho S46) antibody [EP42Y] (ab76242)

  •  
  • Product image

    Anti-p53 antibody [EPR17343] - BSA and Azide free (ab240215)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-p53 antibody [PCRP-TP53-2A10] (ab238066)

    Applications: IHC-P, Protein Array

  •  
  • Product image

    Anti-p53 antibody (ab17990)

    Applications: Flow Cyt, IHC-P, WB

  •  
  • Product image

    Anti-p53 (acetyl K370) antibody [EPR17496] - BSA and Azide free (ab240273)

    Applications: Flow Cyt, ICC/IF, IP, WB

  •  
  • Product image

    Anti-p53 antibody [pAb122] (ab90363)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-p53 antibody (ab224775)

    Applications: IHC-P

  •  
  • Product image

    Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab206054)

    Applications: ICC/IF

  •  
  • Product image

    Anti-p53 antibody [E26] - BSA and Azide free (ab225531)

    Applications: ICC/IF, IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-PRAS40 antibody [EPR6262] (ab134158)

  •  
  • Product image

    Anti-Cyclophilin B antibody [CL3901] (ab236760)

  •  
  • Product image

    Anti-RAB10 antibody [4E2] (ab104859)

  •  
  • Product image

    Anti-BRD2 (phospho S37) antibody [EPR22114] (ab223255)

  •  
  • Product image

    Anti-c-Myc antibody (ab39688)

  •  
  • Product image

    Anti-HOXB13 antibody [mAbcam53931] (ab53931)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.