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Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)

Price and availability

241 228 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5678] to p53 (phospho T377)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-p53 (phospho T377) antibody [EPR5678]
    See all p53 primary antibodies
  • Description

    Rabbit monoclonal [EPR5678] to p53 (phospho T377)
  • Host species

    Rabbit
  • Specificity

    ab133451 only detects p53 phosphorylated at Threonine 377.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human p53 aa 350 to the C-terminus (phospho T377). The exact sequence is proprietary.
    Database link: P04637

  • Positive control

    • Lysate of 293T cells treated with okadaic Acid and calyculin A.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5678
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Images

  • Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/20000 dilution

    Lane 1 : HEK293 (human embryonic kidney) treated with Okadaic acid and Calyculin A whole cell lysates
    Lane 2 : Untreated HEK293 (human embryonic kidney) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM /TBST

  • Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    Western blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/10000 dilution

    Lane 1 : Lysate of 293T cells, untreated
    Lane 2 : Lysate of 293T cells treated with okadaic acid and calyculin A.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat ant-rabbit antibody at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?

  • Dot Blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    Dot Blot - Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: p53 (pT377) phospho peptide

    Lane 2 sample: p53 non-phospho peptide

    Exposure time: 3 minutes

  • Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
    Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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