Anti-p53 (phospho T377) antibody [EPR5678] (ab133451)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5678] to p53 (phospho T377)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho T377) antibody [EPR5678]
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR5678] to p53 (phospho T377) -
Host species
Rabbit -
Specificity
ab133451 only detects p53 phosphorylated at Threonine 377. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human p53 aa 350 to the C-terminus (phospho T377). The exact sequence is proprietary.
Database link: P04637 -
Positive control
- Lysate of 293T cells treated with okadaic Acid and calyculin A.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5678 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/20000 dilution
Lane 1 : HEK293 (human embryonic kidney) treated with Okadaic acid and Calyculin A whole cell lysates
Lane 2 : Untreated HEK293 (human embryonic kidney) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM /TBST
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All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/10000 dilution
Lane 1 : Lysate of 293T cells, untreated
Lane 2 : Lysate of 293T cells treated with okadaic acid and calyculin A.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat ant-rabbit antibody at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: p53 (pT377) phospho peptide
Lane 2 sample: p53 non-phospho peptide
Exposure time: 3 minutes
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