Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [21C10] to Bax - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Bax antibody [21C10] - BSA and Azide free
    See all Bax primary antibodies

  • Description

    Rat monoclonal [21C10] to Bax - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HuT-78, HEK-293T, Raji and HeLa whole cell lysate. Wild type HAP1 whole cell lysate.

  • General notes

    ab252252 is the carrier-free version of ab243140. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252252 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)
    Western blot - Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)
    All lanes : Anti-Bax antibody [21C10] (ab243140) at 1/500 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 2 : BAX knockout HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776)

    Predicted band size: 21 kDa



    This data was produced using the same clone but in a different buffer, ab243140.

    Lanes 1-4: Merged signal (red and green). Green - ab243140 observed at 21 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab243140 Anti-Bax antibody was shown to react with Bax in HAP1 cells in Western blot. Loss of signal was observed when Bax knockout sample was used. Wild-type and Bax knockout samples were subjected to SDS-PAGE.

    ab243140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preadsorbed (ab253031) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Negative control: Jurkat (PMID: 9583678).

    Blocking/Dilution buffer: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBS.

  • Western blot - Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)
    Western blot - Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)
    All lanes : Anti-Bax antibody [21C10] (ab243140) at 1/5000 dilution

    Lane 1 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 21 kDa


    Exposure time: 1 second


    This data was produced using the same clone but in a different buffer, ab243140.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)
    Anti-Bax antibody [21C10] - BSA and Azide free (ab252252)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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