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Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR64(N)] to p53 (phospho S15) - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Dot blot, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR64(N)] to p53 (phospho S15) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Dot blot, ChIPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Doxorubicin treated A431 cells.

  • General notes

    Ab238982 is the carrier-free version of ab223868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238982 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)
    Dot Blot - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

    Dot blot analysis of p53 (phospho S15) labeled with ab223868 at 1/1000 dilution.

    Lane 1: p53 (phospho S15) peptide;

    Lane 2: p53 non-phospho peptide;

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223868).

  • Immunoprecipitation - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)
    Immunoprecipitation - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

    p53 (phospho S15) was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab223868 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab223868 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: A431 treated with 1 μg/ml doxorubicin for 24 hours, whole cell lysate 10 µg (Input). 

    Lane 2: ab223868 IP in A431 treated with 1 μg/ml doxorubicin for 24 hours, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223868 in A431 treated with 1 μg/ml doxorubicin for 24 hours, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223868).

  • ChIP - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)
    ChIP - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

    Chromatin was prepared from HCT 116 (human colorectal carcinoma cell line) cells untreated or treated with 50 µM Etoposide for 6 hours according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. ChIP was performed with 25 mg of chromatin, 5 µg of ab223868 anti-p53 (phospho S15) (blue), and 20 µl of A/G sepharose beads slurry (10 µl of sepharose A beads + 10 µl of sepharose G beads). Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223868).

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

    Immunofluorescent analysis of 4% PFA-fixed A431 (human epidermoid carcinoma cell line) cells labeling p53 (phospho S15) with ab223868 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining on A431 cells treated with 1 μg/ml doxorubicin for 24 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223868).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)
    Anti-p53 (phospho S15) antibody [EPR64(N)] - BSA and Azide free (ab238982)

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