Apoptosis DNA Ladder Assay Kit (ab66090)
Key features and details
- Assay type: Direct
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Apoptosis DNA Ladder Assay Kit
See all DNA fragmentation kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
1h 30m -
Product overview
Apoptosis DNA Ladder Assay Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes. No DNA extraction or additional columns required.
Apoptosis DNA ladder assay protocol summary:
- pellet cells by spinning
- wash cells with PBS and spinning
- lyse cells with TE lysis buffer and pipetting
- add enzyme A solution and incubate for 10 min
- add enzyme B solution and incubate for 30 min
- add ammonium acetate solution, isopropanol and freeze for 10 min
- spin to preciptate DNA
- wash pellet with 70% ethanol, air-dry and dissolve in DNA suspension buffer
- run on agarose gel and visualize with DNA staining dye -
Notes
This product was previously called Apoptotic DNA Ladder Detection Kit.
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 50 tests Ammonium Acetate Solution Yellow 1 x 250µl DNA Suspension Buffer Green 1 x 1.5ml Enzyme A Solution Blue 1 x 250µl Enzyme B (Lyophilized) Red 1 vial TE Lysis Buffer Purple 1 x 1.8ml -
Research areas
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Relevance
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Images
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Functional assays: Apoptotic DNA Ladder Detection Kit (ab66090)
Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.
