Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human RANK Antibody Pair - BSA and Azide free (ab253487)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human RANK Antibody Pair - BSA and Azide free
    See all RANK kits

  • Assay type

    ELISA set

  • Range

    15.625 pg/ml - 1000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    The Antibody Pair can be used to quantify Human RANK. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277714), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human RANK Capture Antibody (unconjugated) 1 x 100µg
    Human RANK Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.

  • Tissue specificity

    Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

  • Involvement in disease

    Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.

  • Sequence similarities

    Contains 4 TNFR-Cys repeats.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • CD 265
    • CD265
    • FEO
    • LOH18CR1
    • Loss of heterozygosity 18 chromosomal region 1
    • mRANK
    • ODFR
    • OFE
    • OPTB7
    • Osteoclast differentiation factor receptor
    • OSTS
    • Paget disease of bone 2
    • PDB 2
    • PDB2
    • RANK
    • Receptor activator of NF KB
    • Receptor activator of NF-KB
    • receptor activator of nuclear factor kappa B
    • TNF receptor superfamily member 11a
    • TNFRSF11A
    • TNR11_HUMAN
    • TRANCER
    • Tumor necrosis factor receptor superfamily member 11A
    • Tumor necrosis factor receptor superfamily member 11a NFKB activator
    • Tumor necrosis factor receptor superfamily member 11a activator of NFKB
    see all

  • Database links

    Images

    • Example of Human RANK standard curve.
      Example of Human RANK standard curve.

      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277714), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253487)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253487)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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