Anti-N Cadherin (phospho Y860) antibody (ab119752)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y860)
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-N Cadherin (phospho Y860) antibody
See all N Cadherin primary antibodies -
Description
Rabbit polyclonal to N Cadherin (phospho Y860) -
Host species
Rabbit -
Specificity
Immunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733). -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseWB Human
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Immunogen
Synthetic phospho-peptide coupled to acarrier protein, corresponding to amino acids surrounding tyrosine 860 in Human N Cadherin.
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Positive control
- C2C12 and Human endothelial cells treated with pervanadate.
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General notes
Do not aliquot.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab119752 was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (ab119752)ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
Detection used an appropriate secondary antibody conjugated to Cy3. -
Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752)Lanes 1-2 : Anti-N Cadherin (Cytoplasmic) at 1/1000 dilution
Lanes 3-4 : Anti-N Cadherin
(phospho Y820) at 1/1000 dilution
Lanes 5-6 : Anti-N Cadherin (a.a. 853-864) at 1/1000 dilution
Lanes 7-8 : Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution
Lanes 1 & 5 & 7 : Human endothelial cells, untreated
Lanes 2 & 4 & 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells, untreated
Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Predicted band size: 99 kDa
