Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E Cadherin antibody [EPR16845-34] (ab212059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16845-34] to E Cadherin
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-E Cadherin antibody [EPR16845-34]
    See all E Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR16845-34] to E Cadherin

  • Host species

    Rabbit

  • Specificity

    E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed.

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse E-Cadherin active protein (aa1-709); Rat spleen lysate; Rat serum; Mouse plasma and serum; Mouse brain lysate. IP: Mouse serum.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Lane 1 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/20000 dilution
    Lane 2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/100000 dilution

    All lanes : Mouse E-Cadherin active protein (aa1-709)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 98 kDa
    Observed band size: 120,84 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Western blot - Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Lanes 1-2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/5000 dilution
    Lanes 3-5 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/1000 dilution

    Lane 1 : Rat spleen lysate
    Lane 2 : Rat serum
    Lane 3 : Mouse plasma
    Lane 4 : Mouse serum
    Lane 5 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 & 5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 98 kDa
    Observed band size: 120,84 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/4/5: 30 seconds; Lane 2: 3 minutes; Lane 3: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; 11953314).

  • Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] (ab212059)

    E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab212059 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab212059 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse serum 10 µg (Input).

    Lane 2: ab212059 IP in mouse serum.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212059 in mouse serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-E Cadherin antibody [EPR16845-34] (ab212059)
    Anti-E Cadherin antibody [EPR16845-34] (ab212059)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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