Anti-Asporin antibody (ab31303)
Key features and details
- Goat polyclonal to Asporin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Asporin antibody
See all Asporin primary antibodies -
Description
Goat polyclonal to Asporin -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide:
C-IHENKVKKIQKDT
, corresponding to Internal sequence amino acids 182-194 of Human Asporin. (Peptide available as ab134013.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Asporin antibody (ab31303) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Asporin antibody (ab31303) at 1/1000 dilution
All lanes : Human osteosarcoma cell line whole lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-goat IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% milk for 1 hour at 27°C.
Incubated with the primary antibody diluted in blocking buffer for 12 hours at 4°C.
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Western blot - Anti-Asporin antibody (ab31303)Anti-Asporin antibody (ab31303) at 0.3 µg/ml + Human Bone Marrow lysate (35µg protein in RIPA buffer)
Predicted band size: 43 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-Asporin antibody (ab31303)Ab31303 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Image shows staining of cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.