Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604)
![Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604)](https://www.abcam.com/ps/products/255/ab255604/Images/ab255604-4-anti-sca1-ly6a-e-antibody-epr22502-68-immunohistochemistry-testis-mouse.jpg "Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22502-68] to Sca1 / Ly6A/E
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-Sca1 / Ly6A/E antibody [EPR22502-68]
See all Sca1 / Ly6A/E primary antibodies -
Description
Rabbit monoclonal [EPR22502-68] to Sca1 / Ly6A/E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse kidney tissue lysate. C2C12 whole cell lysate. IHC-P: Mouse kidney, testis and cardiac muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22502-68 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody (ab255604)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Sca1 / Ly6A/E with ab255604 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody (ab255604)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sca1 / Ly6A/E with ab255604 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody (ab255604)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Sca1 / Ly6A/E with ab255604 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse cardiac muscle (PMID: 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Western blot - Anti-Sca1 / Ly6A/E antibody (ab255604)All lanes : Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 3 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilutionNegative control: EL4 (PMID: 2430016).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 26 secs; Lane 2-3: 5.5 secs.
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![Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604)](https://www.abcam.com/ps/products/255/ab255604/Images/ab255604-5-benefits-of-recombinant-antibodies.png)
Anti-Sca1 / Ly6A/E antibody [EPR22502-68] (ab255604)
