Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Sca1 / Ly6A/E antibody [EPR3355]
    See all Sca1 / Ly6A/E primary antibodies

  • Description

    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse bone marrow, and Hepa1-6 lysates. IHC-P: mouse kidney tissue.

  • General notes

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Sca1 / Ly6A/E with Purified ab109211 at 1:150 dilution (1.37 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/10000 dilution (Purified) + Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution (Purified) + Mouse bone marrow lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

    ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.

    Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
    Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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