Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
Suitable for: WB, IHC-P
Reacts with: Mouse

Product name

Anti-Sca1 / Ly6A/E antibody [EPR3355]
See all Sca1 / Ly6A/E primary antibodies
Description

Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse bone marrow, and Hepa1-6 lysates. IHC-P: mouse kidney tissue.
General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3355
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Sca1 / Ly6A/E with Purified ab109211 at 1:150 dilution (1.37 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/10000 dilution (Purified) + Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?
Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution (Purified) + Mouse bone marrow lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.

Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

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