Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO
Suitable for: ICC/IF, WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Cripto1/CRIPTO antibody [EPNCIR106A]
See all Cripto1/CRIPTO primary antibodies
Description

Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab84062)
Positive control
- WB and IP: NCCIT cell lysate. Flow Cytometry and ICC/IF: NCCIT cells.
General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Cripto1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPNCIR106A
Isotype

IgG
Research areas

Flow Cytometry - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391
at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/2000 dilution (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/1000 dilution (unpurified) + NCCIT cell lysate at 10 µg

Predicted band size: 21 kDa
Observed band size: 21 kDa
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

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