HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)
![HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)](https://www.abcam.com/ps/products/203/ab203468/Images/ab203468-248700-WBab2034681.jpg "HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A]
See all Cripto1/CRIPTO primary antibodies -
Description
HRP Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NCCIT whole cell lysate
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
This product was previously labelled as Cripto1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR106A -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468) at 1/5000 dilution + NCCIT (Human embryonic teratocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203468 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)](https://www.abcam.com/ps/products/203/ab203468/Images/ab203468-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab203468)
