Anti-Cripto1/CRIPTO antibody (ab19917)
Key features and details
- Rabbit polyclonal to Cripto1/CRIPTO
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Cripto1/CRIPTO antibody
See all Cripto1/CRIPTO primary antibodies -
Description
Rabbit polyclonal to Cripto1/CRIPTO -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
-
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23309, ab23310)
Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 100 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23309, ab23310) -
Positive control
- Human Embryonic Stem Cells, Cripto1/CRIPTO Recombinant Protein
-
General notes
This product was previously labelled as Cripto1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab19917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. IHC-P Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa). Target
-
Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
Tissue specificity
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 784029 Cow
- Entrez Gene: 6997 Human
- Entrez Gene: 21667 Mouse
- Omim: 187395 Human
- SwissProt: P13385 Human
- SwissProt: P51865 Mouse
- Unigene: 385870 Human
- Unigene: 5090 Mouse
-
Alternative names
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
Images
-
Western blot - Anti-Cripto1/CRIPTO antibody (ab19917)All lanes : Anti-Cripto1/CRIPTO antibody (ab19917) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
Lane 2 : Cripto1/CRIPTO protein at 0.1 µg
Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml
Lane 4 : Cripto1/CRIPTO protein at 0.1 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 21 kDaThe 17 kDa band detected in human embryonic stem cells may correspond with Cripto1/CRIPTO that has had its signal sequence cleaved. The Cripto1/CRIPTO bands were blocked with the immunizing peptide, ab23310.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody (ab19917)ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody (ab19917)This image is courtesy of Ludovic Vallier, University of Cambridge, UKAnti-Cripto1/CRIPTO antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cripto1/CRIPTO antibody (ab19917)The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto1/CRIPTO protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.
Protocols
References (13)
ab19917 has been referenced in 13 publications.
- Harpelunde Poulsen K et al. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours. BMC Cancer 20:349 (2020). PubMed: 32326899
- Gudbergsson JM & Duroux M An evaluation of different Cripto-1 antibodies and their variable results. J Cell Biochem N/A:N/A (2019). PubMed: 31310365
- Li XS et al. Beneficial effects of Cripto-1 for transarterial chemoembolization in hepatocellular carcinoma. Aging (Albany NY) 11:2998-3011 (2019). PubMed: 31136302
- Lo RC et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/ß-catenin pathway. Cell Death Differ 25:1426-1441 (2018). PubMed: 29445127
- Liu Q et al. Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma. Mol Cancer 16:81 (2017). PubMed: 28431580
Images
-
Western blot - Anti-Cripto1/CRIPTO antibody (ab19917)All lanes : Anti-Cripto1/CRIPTO antibody (ab19917) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
Lane 2 : Cripto1/CRIPTO protein at 0.1 µg
Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml
Lane 4 : Cripto1/CRIPTO protein at 0.1 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 21 kDaThe 17 kDa band detected in human embryonic stem cells may correspond with Cripto1/CRIPTO that has had its signal sequence cleaved. The Cripto1/CRIPTO bands were blocked with the immunizing peptide, ab23310.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody (ab19917)ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody (ab19917) This image is courtesy of Ludovic Vallier, University of Cambridge, UK
Anti-Cripto1/CRIPTO antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cripto1/CRIPTO antibody (ab19917)
The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto1/CRIPTO protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.
