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Developmental Biology Lineage specification Ectoderm

Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody [EPR22230-60]
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT whole cell lysate. IP: NCCIT whole cell lysate.
  • General notes

     This product was previously labelled as Cripto1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22230-60
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Embryonic Stem Cells
    • Secreted
    • Developmental Biology
    • Embryogenesis
    • Morphogens
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)
    Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)

    Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: NCCIT whole cell lysate 10 µg (Input). 

    Lane 2: ab229016 IP in NCCIT whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229016 in NCCIT whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 2 minutes.

  • Western blot - Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)
    Western blot - Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)
    Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016) at 1/1000 dilution + NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The double bands observed are consistent with what has been described in the literature. The lower band represents the immature form of Cripto1/CRIPTO. The upper band is likely to be the processed glycosylated form (PMID: 27881714 and 20568225).

  • Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)
    Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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