Anti-PTPRK antibody (ab13225)
Key features and details
- Rabbit polyclonal to PTPRK
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-PTPRK antibody
See all PTPRK primary antibodies -
Description
Rabbit polyclonal to PTPRK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human PTPRK aa 900-1000 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab13232) -
Positive control
- This antibody gave a positive result in PTP beta (PTPRB) and PTPRK recombinant protein domains
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General notes
This product was previously labelled as PTP kappa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Recombinant fragmentAll applications MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).Target
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Function
Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa. -
Tissue specificity
High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains. -
Post-translational
modificationsThis protein undergoes proteolytic processing. -
Cellular localization
Cell junction > adherens junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5796 Human
- Entrez Gene: 19272 Mouse
- Omim: 602545 Human
- SwissProt: Q15262 Human
- SwissProt: P35822 Mouse
- Unigene: 155919 Human
- Unigene: 332303 Mouse
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Alternative names
- dJ480J14.2.1 antibody
- DKFZp686C2268 antibody
- DKFZp779N1045 antibody
see all
Images
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Western blot - Anti-PTPRK antibody (ab13225)All lanes : Anti-PTPRK antibody (ab13225) at 1 µg/ml
Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
Lane 2 : 0.1µg SAP 1 (PTPRH) protein
Lane 3 : 0.1µg PTP gamma (PTPRG) protein
Lane 4 : 0.1µg PTP beta (PTPRB) protein
Lane 5 : 0.1µg DEP1 (PTPRJ) protein
Lane 6 : 0.1µg PTP kappa (PTPRK) protein
Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
Lane 8 : 0.1µg LAR (PTPRF) protein
Performed under reducing conditions.
Predicted band size: 50-70 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000. The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.
Lane # Recombinant Protein
1 GLEPP1 (PTP RO)
2 SAP 1 (PTP RH)
3 PTP gamma (PTP RG)
4 PTP beta (PTP B)
5  
Datasheets and documents
References (2)
ab13225 has been referenced in 2 publications.
- Agarwal S et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . PubMed: 23696788
- Flavell JR et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). PubMed: 17720884
Images
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Western blot - Anti-PTPRK antibody (ab13225)All lanes : Anti-PTPRK antibody (ab13225) at 1 µg/ml
Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
Lane 2 : 0.1µg SAP 1 (PTPRH) protein
Lane 3 : 0.1µg PTP gamma (PTPRG) protein
Lane 4 : 0.1µg PTP beta (PTPRB) protein
Lane 5 : 0.1µg DEP1 (PTPRJ) protein
Lane 6 : 0.1µg PTP kappa (PTPRK) protein
Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
Lane 8 : 0.1µg LAR (PTPRF) protein
Performed under reducing conditions.
Predicted band size: 50-70 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000. The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.
Lane # Recombinant Protein
1 GLEPP1 (PTP RO)
2 SAP 1 (PTP RH)
3 PTP gamma (PTP RG)
4 PTP beta (PTP B)
5  
