Anti-Anterior Gradient 2 antibody (ab227584)
Key features and details
- Rabbit polyclonal to Anterior Gradient 2
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Anterior Gradient 2 antibody
See all Anterior Gradient 2 primary antibodies -
Description
Rabbit polyclonal to Anterior Gradient 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Anterior Gradient 2 (internal sequence). The exact sequence is proprietary.
Database link: O95994 -
Positive control
- WB: MCF7 whole cell extract. IP: MCF7 whole cell extract. IHC-P: Mouse colon tissue. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody (ab227584)
Paraffin-embedded mouse colon tissue stained for Anterior Gradient 2 with ab227584 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Anterior Gradient 2 antibody (ab227584)Anti-Anterior Gradient 2 antibody (ab227584) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 19 kDa15% SDS-PAGE
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Immunoprecipitation - Anti-Anterior Gradient 2 antibody (ab227584)Anterior Gradient 2 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 μg ab227584. Western blot was performed from the immunoprecipitate using ab227584. Anti-Rabbit IgG was used as the secondary antibody.
Lane 1: MCF7 whole cell extract (Input).
Lane 2: Control IgG IP in MCF7 whole cell extract.
Lane 3: ab227584 IP in MCF7 whole cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody (ab227584)MCF7 (human breast adenocarcinoma cell line) cells stained for Anterior Gradient 2 (green) using ab227584 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
