Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)

Key features and details

  • Mouse monoclonal [9B10D4/4H5E7] to CD34
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-CD34 antibody [9B10D4/4H5E7]
    See all CD34 primary antibodies

  • Description

    Mouse monoclonal [9B10D4/4H5E7] to CD34

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human CD34.

  • Positive control

    • IF: Peripheral blood cells. WB: CD34 recombinant protein

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR182422-21 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)

    ab54208 at 1/1000 dilution staining CD34 in human spleen tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The DAB staining procedure was used for tissue section staining.

  • Flow Cytometry - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Flow Cytometry - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Overlay histogram showing Jurkat cells stained with ab54208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54208, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Western blot - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Anti-CD34 antibody [9B10D4/4H5E7] (ab54208) at 1/200 dilution + Truncated CD34 recombinant protein

    Predicted band size: 41 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [9B10D4/4H5E7] (ab54208)
    Immunofluorescence analysis of peripheral blood cells using ab54208 (1/100) with FITC-IgG.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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