Anti-CD34 antibody [EPR2999] (ab110643)
![Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123952-anti-cd34-antibody-epr2999-immunohistochemistry.jpg "Anti-CD34 antibody [EPR2999] (ab110643)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2999] to CD34
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-CD34 antibody [EPR2999]
See all CD34 primary antibodies -
Description
Rabbit monoclonal [EPR2999] to CD34 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within Human CD34.
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Positive control
- Human bone marrow tissue lysate TF-1 cell lysate Human kidney tissue and Human tonsil tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2999 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)
ab110643 showing positive staining in Normal stomach vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123955-anti-cd34-antibody-epr2999-western-blot.jpg)
Western blot - Anti-CD34 antibody [EPR2999] (ab110643)All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution
Lane 1 : Human bone marrow tissue lysate
Lane 2 : TF-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123951-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal placenta vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123950-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal lung vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123949-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643 showing positive staining in Normal breast vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123954-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-123953-anti-cd34-antibody-epr2999-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CD34 antibody [EPR2999] (ab110643)](https://www.abcam.com/ps/products/110/ab110643/Images/ab110643-12-benefits-of-recombinant-antibodies.png)
Anti-CD34 antibody [EPR2999] (ab110643)
