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Anti-CREB antibody [LB9] (ab178322)

Key features and details

  • Mouse monoclonal [LB9] to CREB
  • Suitable for: ICC/IF, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-CREB antibody [LB9]
    See all CREB primary antibodies

  • Description

    Mouse monoclonal [LB9] to CREB

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human CREB aa 1-341.
    Sequence:

    MTMESGAENQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA PTVTLVQLPN GQTVQVHGVI QAAQPSVIQS PQVQTVQSSC KDLKRLFSGT QISTIAESED SQESVDSVTD SQKRREILSR RPSYRKILND LSSDAPGVPR IEEEKSEEET SAPAITTVTV PTPIYQTSSG QYIAITQGGA IQLANNGTDG VQGLQTLTMT NAAATQPGTT ILQYAQTTDG QQILVPSNQV VVQAASGDVQ TYQIRTAPTS TIAPGVVMAS SPALPTQPAE EAARKREVRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK ALKDLYCHKS D


    Database link: P16220
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa cells; Human bladder and rectal cancer tissues; Human tonsil tissue; Human HeLa, 293T, HepG2, A431, A549, K562, MCF7 and U2Os whole cell lysates; Mouse MEF, 3T3L1 and C2C12 whole cell lyastes; Rat NRK whole cell lysate; Monkey COS7 whole cell lysate.

Properties

Images

  • Western blot - Anti-CREB antibody [LB9] (ab178322)
    Western blot - Anti-CREB antibody [LB9] (ab178322)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CREB knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab178322 observed at 44 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab178322 was shown to recognize CREB when CREB knockout samples were used, along with additional cross-reactive bands. Wild-type and CREB knockout samples were subjected to SDS-PAGE.  Ab178322 and ab18058 (loading control to Vinculin) were diluted at 1/250 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CREB antibody [LB9] (ab178322)
    Western blot - Anti-CREB antibody [LB9] (ab178322)
    All lanes : Anti-CREB antibody [LB9] (ab178322) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : A549 whole cell lysate
    Lane 5 : MEF whole cell lysate
    Lane 6 : MCF7 whole cell lysate
    Lane 7 : U2OS whole cell lysate
    Lane 8 : K562 whole cell lysate
    Lane 9 : COS7 whole cell lysate
    Lane 10 : 3T3L1 whole cell lysate
    Lane 11 : C2C12 whole cell lysate
    Lane 12 : NRK whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [LB9] (ab178322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [LB9] (ab178322)

    Immunohistochemical analysis of deparaffinized Human tonsil tissue labeling CREB with ab178322 at 1/100 dilution followed by DAB staining.

  • Immunoprecipitation - Anti-CREB antibody [LB9] (ab178322)
    Immunoprecipitation - Anti-CREB antibody [LB9] (ab178322)

    Immunoprecipitation analysis of CREB was performed on untreated 293T cells. The antigen:antbody complex was formed by binding 500μg whole cell lysate with 2μg ab178322 and captured on 50μl Protein A/G Plus Agarose. 

    Lane 1: 293T lysate

    Lane 2: IP of 293T lysate

    WB detection used ab178322 at 1/1000 followed with a goat anti-mouse-HRP secondary antibody at 1/20,000.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [LB9] (ab178322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [LB9] (ab178322)

    Immunohistochemical analysis of deparaffinized Human bladder cancer (left) and rectal cancer (right) tissues labeling CREB with ab178322 at 1/200 dilution followed by DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB antibody [LB9] (ab178322)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB antibody [LB9] (ab178322)

    Immunofluorescent analysis of untreated HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling CREB with ab178322 at 1/400 dilution (green) followed with DyLight 488 goat anti-mouse IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB antibody [LB9] (ab178322)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB antibody [LB9] (ab178322)

    Immunofluorescent analysis of untreated HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling CREB with ab178322 at 1/500 dilution (green) followed with DyLight 488 goat anti-mouse IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

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