Anti-Twist antibody [10E4E6] (ab175430)
Key features and details
- Mouse monoclonal [10E4E6] to Twist
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Twist antibody [10E4E6]
See all Twist primary antibodies -
Description
Mouse monoclonal [10E4E6] to Twist -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB MouseHumanRecombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
Sequence:PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR
Database link: Q15672 -
Positive control
- Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabiliser. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
10E4E6 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Raji cell lysate
Lane 6 : OCM-1 cell lysate
Predicted band size: 21 kDa
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
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Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
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All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa
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Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein
Predicted band size: 21 kDaExpected MW is 31.9 kDa.