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Neuroscience Cell Type Marker Neuron marker Soma marker

Anti-Twist antibody [10E4E6] (ab175430)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Twist antibody [10E4E6] (ab175430)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [10E4E6] to Twist
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Twist antibody [10E4E6]
    See all Twist primary antibodies
  • Description

    Mouse monoclonal [10E4E6] to Twist
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
    Sequence:

    PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR


    Database link: Q15672
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabiliser.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    10E4E6
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle

Images

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : OCM-1 cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
    Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)

    Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
    Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)

    Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein

    Predicted band size: 21 kDa



    Expected MW is 31.9 kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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