Anti-Twist (phospho S68) antibody [EP18178] (ab187008)
Key features and details
- Rabbit monoclonal [EP18178] to Twist (phospho S68)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Twist (phospho S68) antibody [EP18178]
See all Twist primary antibodies -
Description
Rabbit monoclonal [EP18178] to Twist (phospho S68) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate. IP: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP18178 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Twist (phospho S68) antibody [EP18178] (ab187008) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
PMID: 21502402.
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Dot blot analysis of Twist (phospho S68) labeled with ab187008 at 1/1000 dilution.
Lane 1: Twist (phospho S68) peptide;
Lane 2: Twist non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Twist (phospho S68) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate with ab187008 at 1/60 dilution.
Western blot was performed from the immunoprecipitate using ab187008 at 1/5000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate 10ug (Input).
Lane 2: ab187008 IP in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187008 in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.