Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR056F] to CD40 - BSA free
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-CD40 antibody [BLR056F] - BSA free
See all CD40 primary antibodies
Description

Rabbit monoclonal [BLR056F] to CD40 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD40 aa 227-277. The exact sequence is proprietary. NP_001241.1
Database link: P25942-1
Positive control
- IHC-P: Human breast carcinoma and tonsil tissue. ICC: Raji cells. IP: Raji whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272087 is the carrier-free version of ab252557. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR056F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252557 used for IP at 5 µl per reaction. For WB 1/1000 dilution.
Lane 1: ab252557 IP in Raji whole cell lysate.
Lane 2: Control IgG IP in Raji whole cell lysate.
Chemiluminescence detection: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).
Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using ab252557 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).
Anti-CD40 antibody [BLR056F] - BSA free (ab272087)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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