Human LAG3 ELISA Kit (ab193707)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 45 pg/well - 12000 pg/well
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LAG3 ELISA Kit
See all LAG-3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
45 pg/well - 12000 pg/well -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.4 70% - 86% Plasma 78.2 68% - 95% Cell culture media 76.83 69% - 85% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s LAG3 (lymphocyte activation gene 3) Human ELISA Kit (ab193707) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAG3 in serum, plasma and cell culture supernatant. (UniprotID: P18627)
This assay employs an antibody specific for human LAG3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures LAG3 present in the samples. The wells are washed and biotinylated anti-human LAG3 is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of LAG3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent D 1 x 15ml 5X Assay Diluent B 1 x 15ml Biotinylated Human LAG3 detection antibody (lyophilized) 2 vials Human LAG3 Standards (lyophilized) 2 vials Pre-coated Human LAG3 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD223
- CD223 antigen
- FDC protein
see all -
Database links
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
Images
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Typical standard curve
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.