Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Twist antibody [Twist2C1a] (ab50887)

Key features and details

  • Mouse monoclonal [Twist2C1a] to Twist
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Twist antibody [Twist2C1a]
    See all Twist primary antibodies

  • Description

    Mouse monoclonal [Twist2C1a] to Twist

  • Host species

    Mouse

  • Specificity

    Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Twist (C terminal).

  • Positive control

  • General notes

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)
    Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)
    All lanes : Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/ml

    Lane 1 : A2058 (Human melanoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : F2408 (Rat normal fibroblast cell line) whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 21 kDa

  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)
    Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

    4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 µg/ml in immunocytochemistry.

  • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) This image is courtesy of an Abreview submitted by Pawel Mazur
    All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C

    Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
    Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma

    Lysates at 50 µg per lane

    Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) Loebel DA et al., PLoS One, 9, e98945, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody

  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)

    ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat anti-mouse IgG at 1/200 dilution.

  • Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Western blot - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Lanes 2-5 : Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution

    Lane 1 : Marker lane
    Lane 2 : Hela whole cell lysate, 25µg
    Lane 3 : A2058 whole cell lysate, 25µg
    Lane 4 : NIH3T3 whole cell lysate, 25µg
    Lane 5 : F2408 whole cell lysate, 25µg

    Predicted band size: 21 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    SDS-PAGE with 10-20% gradient gel.
  • Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)
    Immunocytochemistry - Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) This image is courtesy of an anonymous Abreview

    ab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.

    See Abreview

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