Anti-c-Fos antibody [EPR883(2)] (ab134122)
![Anti-c-Fos antibody [EPR883(2)] (ab134122)](https://www.abcam.com/ps/products/134/ab134122/Images/ab134122-148339-anti-c-fos-antibody-epr8832-western-blot.jpg "Anti-c-Fos antibody [EPR883(2)] (ab134122)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR883(2)] to c-Fos
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-c-Fos antibody [EPR883(2)]
See all c-Fos primary antibodies -
Description
Rabbit monoclonal [EPR883(2)] to c-Fos -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human c-Fos aa 200-300. The exact sequence is proprietary.
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Positive control
- Lysates of U937 cells treated with TPA and untreated U937 cells, Lysate of HeLa cells treated with TPA.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR883(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Fos antibody [EPR883(2)] (ab134122)All lanes : Anti-c-Fos antibody [EPR883(2)] (ab134122) at 1/1000 dilution
Lane 1 : Lysate of U937 cells treated with TPA
Lane 2 : U937 cell lysate
Lane 3 : Lysate of HeLa cells treated with TPA
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-c-Fos antibody [EPR883(2)] (ab134122)](https://www.abcam.com/ps/products/134/ab134122/Images/ab134122-215926-anti-c-fos-antibody-epr8832-flow-cytometry.jpg)
Flow Cytometry - Anti-c-Fos antibody [EPR883(2)] (ab134122)Overlay histogram showing HeLa cells stained with ab134122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134122, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Anti-c-Fos antibody [EPR883(2)] (ab134122)](https://www.abcam.com/ps/products/134/ab134122/Images/ab134122-3-benefits-of-recombinant-antibodies.png)
Anti-c-Fos antibody [EPR883(2)] (ab134122)
