Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-c-Fos antibody [EPR21930-238] (ab222699)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21930-238] to c-Fos
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Human

Product name

Anti-c-Fos antibody [EPR21930-238]
See all c-Fos primary antibodies
Description

Rabbit monoclonal [EPR21930-238] to c-Fos
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat and RAW 264.7 grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysates. IHC-P: Human bladder carcinoma tissue; Mouse dentate gryus and cerebral cortex tissues. ICC/IF: HeLa cells. Flow: HeLa cells. IP: HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21930-238
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR21930-238] (ab222699)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling c-Fos with ab222699 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neurons of mouse cerebral cortex (PMID: 24604295). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-c-Fos antibody [EPR21930-238] (ab222699)

c-Fos was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours whole cell lysate with ab222699 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222699 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours whole cell lysate 10 μg (Input).

Lane 2: ab222699 IP in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222699 in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 30 seconds.

Serum starvation followed by FBS treatment induces the expression of c-Fos (PMID: 14981092).
Flow Cytometry - Anti-c-Fos antibody [EPR21930-238] (ab222699)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permebalized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours (Red) / Untreated control (Green) labeling c-Fos with ab22699 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

Serum starvation followed by FBS treatment induces the expression of c-Fos (PMID: 14981092).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR21930-238] (ab222699)

Immunohistochemical analysis of paraffin-embedded mouse dentate gyrus tissue labeling c-Fos with ab222699 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic nuclear staining in mouse dentate gyrus (PMID: 24604295). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR21930-238] (ab222699)

Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling c-Fos with ab222699 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in tumor cells of human bladder carcinoma (PMID: 28358415). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR21930-238] (ab222699)

Immunofluorescnt analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeablised HeLa (human cervix adenocarcinoma epithelial cell) cells labeling c-Fos with ab222699 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cells in serum free medium for 36 hours, followed by addition of 20% fetal bovine serum for 2 hours (PMID: 14981092).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-c-Fos antibody [EPR21930-238] (ab222699)

All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) grown in serum free medium overnight, whole cell lysate
Lane 2 : RAW 264.7 grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 41 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
Western blot - Anti-c-Fos antibody [EPR21930-238] (ab222699)

All lanes : Anti-c-Fos antibody [EPR21930-238] (ab222699) at 1/1000 dilution

Lane 1 : Untreated Jurkat (human T cell leukemia cell line from peripheral blood) grown in serum free medium overnight, whole cell lysate
Lane 2 : Jurkat grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 41 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
Anti-c-Fos antibody [EPR21930-238] (ab222699)

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