Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
![Anti-c-Fos antibody [7D6-A9-D4] (ab184666)](https://www.abcam.com/ps/products/184/ab184666/Images/ab184666-336913-anti-c-fos-antibody-7d6-a9-d4-western-blot.jpg "Anti-c-Fos antibody [7D6-A9-D4] (ab184666)")
Key features and details
- Mouse monoclonal [7D6-A9-D4] to c-Fos
- Suitable for: WB
- Reacts with: Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-c-Fos antibody [7D6-A9-D4]
See all c-Fos primary antibodies -
Description
Mouse monoclonal [7D6-A9-D4] to c-Fos -
Host species
Mouse -
Specificity
This antibody detects endogenous levels of c-Fos and does not cross-react with related proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human, African green monkey -
Immunogen
Recombinant fragment within Human c-Fos. The exact sequence is proprietary. Purified recombinant human c-Fos protein fragments expressed in E.coli. NM_005252.
Database link: P01100 -
Positive control
- HeLa, Cos7, HCT116 and C6 cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 03/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Concentrated Culture Supernatant -
Purification notes
Concentrated cell culture supernatant -
Clonality
Monoclonal -
Clone number
7D6-A9-D4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/1000 dilution
Lane 1 : COS7
Lane 2 : HCT116
Predicted band size: 62 kDa
Observed band size: 62 kDa
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![Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)](https://www.abcam.com/ps/products/184/ab184666/Images/ab184666-215641-anti-c-fos-antibody-7d6-a9-d4-western-blot.jpg)
Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution
Lane 1 : HeLa cell lysate without TSA treatment
Lane 2 : HeLa cell lysate with TSA treatment
Predicted band size: 62 kDaThis image was generated using the ascites version of the product.
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![Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)](https://www.abcam.com/ps/products/184/ab184666/Images/ab184666-215640-anti-c-fos-antibody-7d6-a9-d4-western-blot.jpg)
Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with 50 uM TPA
Lane 3 : HeLa cell lysate treated with 50 uM TSA
Predicted band size: 62 kDaThe lower panel is labeled with anti-GAPDH at 1/1000 dilution.
This image was generated using the ascites version of the product.
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![Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)](https://www.abcam.com/ps/products/184/ab184666/Images/ab184666-215639-anti-c-fos-antibody-7d6-a9-d4-western-blot.jpg)
Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution
Lane 1 : HCT116 cell lysate
Lane 2 : Cos7 cell lysate
Lane 3 : C6 cell lysate
Predicted band size: 62 kDaThis image was generated using the ascites version of the product.
