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Anti-c-Fos antibody [7D6-A9-D4] (ab184666)

Key features and details

  • Mouse monoclonal [7D6-A9-D4] to c-Fos
  • Suitable for: WB
  • Reacts with: Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-c-Fos antibody [7D6-A9-D4]
    See all c-Fos primary antibodies

  • Description

    Mouse monoclonal [7D6-A9-D4] to c-Fos

  • Host species

    Mouse

  • Specificity

    This antibody detects endogenous levels of c-Fos and does not cross-react with related proteins.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Rat, Human, African green monkey

  • Immunogen

    Recombinant fragment within Human c-Fos. The exact sequence is proprietary. Purified recombinant human c-Fos protein fragments expressed in E.coli. NM_005252.
    Database link: P01100

  • Positive control

    • HeLa, Cos7, HCT116 and C6 cell lysates.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 03/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team

Properties

Images

  • Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/1000 dilution

    Lane 1 : COS7
    Lane 2 : HCT116

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HeLa cell lysate without TSA treatment
    Lane 2 : HeLa cell lysate with TSA treatment

    Predicted band size: 62 kDa



    This image was generated using the ascites version of the product.

  • Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with 50 uM TPA
    Lane 3 : HeLa cell lysate treated with 50 uM TSA

    Predicted band size: 62 kDa



    The lower panel is labeled with anti-GAPDH at 1/1000 dilution.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    Western blot - Anti-c-Fos antibody [7D6-A9-D4] (ab184666)
    All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HCT116 cell lysate
    Lane 2 : Cos7 cell lysate
    Lane 3 : C6 cell lysate

    Predicted band size: 62 kDa



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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