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Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20769] to c-Fos - BSA and Azide free
  • Suitable for: IP, ICC/IF, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-c-Fos antibody [EPR20769] - BSA and Azide free
    See all c-Fos primary antibodies

  • Description

    Rabbit monoclonal [EPR20769] to c-Fos - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Human c-Fos aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P01100

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: Serum treated HeLa cells.

  • General notes

    Ab236039 is the carrier-free version of ab214672. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236039 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)
    Immunoprecipitation - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)

    c-Fos was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab214672 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214672 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at a 1/1000 diliution.
    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20%FBS for 2 hours, whole cell lysate, 10 μg (Input).
    Lane 2: ab214672 IP in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214672 in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    The observed lower band is a proteasomal degradation fragment (PMID: 9737957).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214672).

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)
    Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized serum treated and non-treated HeLa (human cervix adenocarcinoma epithelial cell) cells labeling c-Fos with ab214672 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing weakly nuclear staining on HeLa cells grown in serum free medium for 36 hours. Expression of c-Fos increased in HeLa cells grown in serum free medium for 36 hours followed by addition of 20% FBS for 2 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214672).

  • Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)
    Anti-c-Fos antibody [EPR20769] - BSA and Azide free (ab236039)

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