Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4681] to RENT1/hUPF1 - BSA and Azide free
Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-P
Reacts with: Mouse, Human

Product name

Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free
See all RENT1/hUPF1 primary antibodies
Description

Rabbit monoclonal [EPR4681] to RENT1/hUPF1 - BSA and Azide free
Host species

Rabbit
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human kidney, liver and transitional cell carcinoma tissue, Flow Cyt: HeLa cells. ICC/IF: HeLa cells. IP: Raji cells.
General notes
ab243911 is the carrier-free version of ab109363 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab243911 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4681
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RENT1/hUPF1 with Purified ab109363 at 1:100 dilution (5.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)
Immunoprecipitation - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

ab109363 (purified) at 1:30 dilution (2µg) immunoprecipitating RENT1/hUPF1 in Raji whole cell lysate.

Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab109363 & Raji whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109363 in Raji whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling RENT1/hUPF1 with Purified ab109363 at 1:100 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)
Flow Cytometry - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RENT1/hUPF1 with Purified ab109363 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)
Immunocytochemistry/ Immunofluorescence - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling RENT1/hUPF1 with purified ab109363 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

ab109363, at 1/100, staining RENT1/hUPF1 in Human kidney tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)

This image was generated using the unpurified version of the product.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

ab109363, at 1/100, staining RENT1/hUPF1 in Human transitional cell carcinoma tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

ab109363, at 1/100, staining RENT1/hUPF1 in HeLa cells by immunofluorescence.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109363)

This image was generated using the unpurified version of the product.
Anti-RENT1/hUPF1 antibody [EPR4681] - BSA and Azide free (ab243911)

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