Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRGX antibody [EPR9775(2)] (ab178405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9775(2)] to MRGX
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MRGX antibody [EPR9775(2)]
    See all MRGX primary antibodies

  • Description

    Rabbit monoclonal [EPR9775(2)] to MRGX

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human MRGX aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15014

  • Positive control

    • K562, HeLa, A431 and U87-MG cell lysates; Human ovarian carcinoma and breast tissues; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    All lanes : Anti-MRGX antibody [EPR9775(2)] (ab178405) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.

  • Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.

  • Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Anti-MRGX antibody [EPR9775(2)] (ab178405)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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