Anti-RENT1/hUPF1 antibody (ab86057)
Key features and details
- Rabbit polyclonal to RENT1/hUPF1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RENT1/hUPF1 antibody
See all RENT1/hUPF1 primary antibodies -
Description
Rabbit polyclonal to RENT1/hUPF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region between residues 1079 and 1129 of human RENT1/hUPF1 (NP_002902.2)
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Positive control
- HeLa and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab86057 was affinity purified using an epitope specific to RENT1/hUPF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RENT1/hUPF1 antibody (ab86057)All lanes : Anti-RENT1/hUPF1 antibody (ab86057) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-RENT1/hUPF1 antibody (ab86057)Detection of RENT1/hUPF1 by Western Blot of Immunprecipitate.
ab86057 at 1µg/ml staining RENT1/hUPF1 in HeLa whole cell lysate immunoprecipitated using ab86057 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
