Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸ 100 µl 334 369 ₸

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HRP Anti-RENT1/hUPF1 antibody [EPR4681] (ab201818)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR4681] to RENT1/hUPF1
Suitable for: WB, IHC-P
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-RENT1/hUPF1 antibody [EPR4681]
See all RENT1/hUPF1 primary antibodies
Description

HRP Rabbit monoclonal [EPR4681] to RENT1/hUPF1
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HuT 78, Raji cell lysates. IHC-P: FFPE human normal testis tissue sections.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4681
Isotype

IgG
Research areas

Western blot - HRP Anti-RENT1/hUPF1 antibody [EPR4681] (ab201818)

All lanes : HRP Anti-RENT1/hUPF1 antibody [EPR4681] (ab201818) at 1/1000 dilution

Lane 1 : HuT 78 Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 124 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

Exposure time: 4 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201818 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RENT1/hUPF1 antibody [EPR4681] (ab201818)

IHC image of RENT1/hUPF1 staining in a section of formalin-fixed paraffin-embedded human normal testis*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201818, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
HRP Anti-RENT1/hUPF1 antibody [EPR4681] (ab201818)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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