Human Thymidine Kinase 1 ELISA Kit (ab223595)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.1 pg/ml
- Range: 23.5 pg/ml - 3500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thymidine Kinase 1 ELISA Kit
See all Thymidine Kinase 1/TK1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 5 3.3% Inter-assay Sample n Mean SD CV% Cell Extract 3 7.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.1 pg/ml -
Range
23.5 pg/ml - 3500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 95% - 104% Serum 85 75% - 91% Cell culture extracts 102 86% - 120% EDTA Plasma 80 80% - 81% Cit plasma 80 75% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Thymidine Kinase 1 ELISA Kit (ab223595) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thymidine Kinase 1 protein in cell culture supernatant, cit plasma, edta plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thymidine Kinase 1 with 6.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Thymidine kinase 1, or TK1, is a 234- amino acid, zinc binding protein encoded by the Thymidine Kinase 1 gene. The primary function of Thymidine Kinase 1 is the phosphorylation of thymidine to form deoxythymidine 5’-phosphate, dTMP, and is necessary for DNA replication. The activity of Thymidine Kinase 1 is highest during the S phase of cells, and lowers during the G2 phase. Two forms of Thymidine Kinase 1 have been identified, one in the cytosol and the other in mitochondria, which is known as TK2. The presence of Thymidine Kinase 1 in serum may be a biomarker for certain types of cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human Thymidine Kinase 1 Capture Antibody 1 x 600µl 10X Human Thymidine Kinase 1 Detector Antibody 1 x 600µl Human Thymidine Kinase 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 50ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the thymidine kinase family. -
Post-translational
modificationsPhosphorylated on Ser-13 in mitosis. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- cytosolic
- KITH_HUMAN
- Thymidine kinase
see all -
Database links
- Entrez Gene: 7083 Human
- Omim: 188300 Human
- SwissProt: P04183 Human
- Unigene: 515122 Human
Images
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Other - Human Thymidine Kinase 1 ELISA Kit (ab223595)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Thymidine Kinase 1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Thymidine Kinase 1 standard curve in Sample Diluent 50BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Thymidine Kinase 1 in human PC3, MCF7, and SH-SY5Y cell extracts.Interpolated concentrations of native Thymidine Kinase 1 in human PC-3, MCF7, and SH-SY5Y cell extracts based on a 16 μg/mL extract load. The concentrations of Thymidine Kinase 1 were measured in duplicate and interpolated from the Thymidine Kinase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thymidine Kinase 1 concentration was determined to be 1251 pg/ml in PC-3, 585 pg/mL in MCF7, 721 pg/mL in SH-SY5Y.
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Interpolated concentrations of spiked Thymidine Kinase 1 in human serum, plasma and cell culture supernatant samples.The concentrations of Thymidine Kinase 1 were measured in duplicates, interpolated from the Thymidine Kinase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 25% and HUVEC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human Thymidine Kinase 1 ELISA Kit (ab223595)To learn more about the advantages of recombinant antibodies see here.
