Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COMT antibody [EPR6491(B)] (ab124813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6491(B)] to COMT
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-COMT antibody [EPR6491(B)]
    See all COMT primary antibodies

  • Description

    Rabbit monoclonal [EPR6491(B)] to COMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human COMT aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293T, HAP1, A431 and MOLT4 cell lysates; Human fetal liver lysate. Flow Cyt: A431 cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : COMT knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab124813 Anti-COMT antibody [EPR6491(B)] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab124813 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : COMT knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 30 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
    All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MOLT4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 30 kDa

  • Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
    Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
    ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
  • OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)
    OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-COMT antibody [EPR6491(B)] (ab124813)
    Anti-COMT antibody [EPR6491(B)] (ab124813)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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