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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-WDR66 antibody [2A6F7] (ab175369)

Anti-WDR66 antibody [2A6F7] (ab175369)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2A6F7] to WDR66
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-WDR66 antibody [2A6F7]
  • Description

    Mouse monoclonal [2A6F7] to WDR66
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human WDR66 aa 1-250. Expressed in E. Coli.
    Sequence:

    MSDAAEAPRE ATGENGETEM KEEEEPNPNY KEVEDPQQES KDDTIAWRES QEEERKTGEE EGEEEGKEDK KIVMEETEEK AGEVQEKEAS GIQEETTVEP QEVTASMIRL ETQITDSQSI TSGIFPKTQR GSKSKLSLQL EDAETDELLR DLSTQIEFLD LDQISPEEQQ ISSPERQPSG ELEEKTDRMP QDELGQERRD LEPENREEGQ ERRVSDIQSK AGISRESLVS STTEDILFQK DKSTPVYPLT


    Database link: Q8TBY9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human WDR66 recombinant protein; WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate; HepG2 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2A6F7
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other

Images

  • Western blot - Anti-WDR66 antibody [2A6F7] (ab175369)
    Western blot - Anti-WDR66 antibody [2A6F7] (ab175369)
    All lanes : Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution

    Lane 1 : Non transfected HEK293 cell lysate
    Lane 2 : WDR66 (aa: 1-250)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 130 kDa



    Expected MW is 53.9 kDa

  • Western blot - Anti-WDR66 antibody [2A6F7] (ab175369)
    Western blot - Anti-WDR66 antibody [2A6F7] (ab175369)
    Anti-WDR66 antibody [2A6F7] (ab175369) at 1/500 dilution + Human WDR66 recombinant protein

    Predicted band size: 130 kDa



    Expected MW is 53.9 kDa

  • Flow Cytometry - Anti-WDR66 antibody [2A6F7] (ab175369)
    Flow Cytometry - Anti-WDR66 antibody [2A6F7] (ab175369)

    Flow cytometric analysis of HepG2 cells labeling WDR66 using ab175369 at 1/200 dilution (green) and negative control (purple).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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