Anti-RENT1/hUPF1 antibody [EP4682] (ab133564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4682] to RENT1/hUPF1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RENT1/hUPF1 antibody [EP4682]
See all RENT1/hUPF1 primary antibodies -
Description
Rabbit monoclonal [EP4682] to RENT1/hUPF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human RENT1/hUPF1 aa 1000-1100 (internal sequence). The exact sequence is proprietary.
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Positive control
- Human kidney tissue; HuT-78, Raji, SH-SY5Y and HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP4682 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RENT1/hUPF1 antibody [EP4682] (ab133564) at 1/10000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 124 kDa
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RENT1/hUPF1 with ab133564 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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