11,12 EET / DHET ELISA Kit (ab175815)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 0.01 ng/ml - 1000 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
11,12 EET / DHET ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall 12 0.21% Inter-assay Sample n Mean SD CV% overall 12 1.82% -
Sample type
Urine, Serum, Plasma, Cell culture extracts, Tissue -
Assay type
Competitive -
Sensitivity
10 pg/ml -
Range
0.01 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s 11,12 EET / DHET in vitro ELISA kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture, tissue samples following proper isolation and purification.
A 96-well plate has been precoated with 11,12 DHET antibody. Samples and the 11,12 DHET-HRP conjugate are added to the wells, where 11,12 DHET in the sample competes with the added 11,12 DHET-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 11,12 DHET in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 11,12-DHET Standard 1 x 2µl 11,12-DHET-HRP Conjugates 1 x 12µl HRP Buffer 1 x 15ml Pre-coated 96-Well Microplate 1 unit 10X Sample Dilution Buffer 1 x 25ml TMB Substrate 1 x 24ml 10X Wash Buffer Solution 1 x 25ml -
Research areas
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Alternative names
- 11,12-dihydroxy-5Z,8Z,14Z-eicosatrienoic acid
Images
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Typical Standard Curve
Representative standard curve using ab175815 11,12 EET / DHET ELISA Kit.