ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol

Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 µg/ml).

ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An  Alexa Fluor®488-conjugated Donkey anti-goat  polyclonal(1/500) was used as the secondary antibody.

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