Human Vitronectin/S-Protein ELISA Kit (ab234577)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19.9 pg/ml
- Range: 0.06 ng/ml - 4 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Vitronectin/S-Protein ELISA Kit
See all Vitronectin/S-Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rec protein 6 3% -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
19.9 pg/ml -
Range
0.06 ng/ml - 4 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114.9 107% - 119.2% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Vitronectin/S-Protein Capture Antibody 1 x 600µl 10X Human Vitronectin/S-Protein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Vitronectin/S-Protein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
Tissue specificity
Plasma. -
Sequence similarities
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
Post-translational
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Complement S Protein
- Epibolin
- S Protein
see all -
Database links
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
Images
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Other - Human Vitronectin/S-Protein ELISA Kit (ab234577)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.The Vitronectin standard curve was prepared as described in Section 10.
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Interpolated concentrations of native Vitronectin in human serum samples.The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 µg/mL in serum.
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Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (ab234577)To learn more about the advantages of recombinant antibodies see here.