Anti-4E-T antibody (ab55881)
Key features and details
- Rabbit polyclonal to 4E-T
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-4E-T antibody
See all 4E-T primary antibodies -
Description
Rabbit polyclonal to 4E-T -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human 4E-T aa 1-19 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:MDRRSMGETESGDAFLDLK-C
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Positive control
- HeLa cell lysates HEK-293T cells
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General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-4E-T antibody (ab55881)Lanes 1 & 3-4 : Anti-4E-T antibody (ab55881) at 1 µg/ml
Lane 2 : Anti-4E-T antibody (ab55881) at 2 µg/ml
Lanes 1-2 : Extracts of HeLa cells
Lane 3 : Extracts of HeLa cells with 4E-T immunizing peptide at 10 µg/ml
Lane 4 : Extracts of HeLa cells with Non-relevant peptide
at 10 µg/ml
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 108 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-4E-T antibody (ab55881)IF localization of 4E-T in HEK-293T cells stained with ab55881 at 5 mg/ml (A), and DAPI (B). The antibody was detected with a FITC conjugated Goat anti-Rabbit IgG.
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Western blot - Anti-4E-T antibody (ab55881) Image courtesy of an anonymous Abreview.All lanes : Anti-4E-T antibody (ab55881) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HeLa cells
Lane 2 : siRNA knockdown
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 800CW conjugated goat anti-rabbit polyclonal at 1/15000 dilution
Predicted band size: 108 kDa