Human M-CSF ELISA Kit (ab245714)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.8 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human M-CSF ELISA Kit
See all M-CSF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 8 6.5% Inter-assay Sample n Mean SD CV% plasma 3 9.9% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.8 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 87% - 106% Saliva 99 98% - 101% Urine 92 85% - 97% Serum 117 114% - 122% Hep Plasma 110 107% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human M-CSF ELISA Kit (ab245714) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of M-CSF protein in hep plasma, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human M-CSF with 3.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human M-CSF Capture Antibody 1 x 600µl 10X Human M-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human M-CSF Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
Post-translational
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
Database links
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
Images
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Other - Human M-CSF ELISA Kit (ab245714)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human M-CSF Standard Curve Comparison.Standard Curve comparison between human M-CSF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human M-CSF standard curve in Sample Diluent NS.The M-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native M-CSF in human serum, plasma (heparin), and PHA-M stimulated PBMC cell culture supernatant samples.The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (heparin) 2.5%, and PBMC supernatant 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 11.5 ng/mL in serum, 6.7 ng/mL in plasma (heparin), and 2.4 ng/mL in stimulated PBMC supernatant. -
Interpolated concentrations of native M-CSF in human saliva, urine, and A549 cell supernatant samples.The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 25%, urine 1:200, and A549 cell supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 0.9 ng/mL in saliva, 28.1 ng/mL in urine, and 0.89 ng/mL in A549 cell supernatant. -
Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 27.8 ng/mL with a range of 17.7 - 47.2 ng/mL.
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Sandwich ELISA - Human M-CSF ELISA Kit (ab245714)To learn more about the advantages of recombinant antibodies see here.
